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antibodies against cd86  (Elabscience Biotechnology)


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    Elabscience Biotechnology antibodies against cd86
    Antibodies Against Cd86, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibodies+against+cd86/APC+Anti-Mouse+CD86+Antibody/pmc13059438-184-10-13
    Average 95 stars, based on 93 article reviews
    antibodies against cd86 - by Bioz Stars, 2026-10
    95/100 stars

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    other:

    Article Title: Modulating the Immunosuppressive Tumor Microenvironment and Inhibiting Growth in Mutp53-Driven CRPC via STAT3 Pathway Blockade.
    Article Snippet: Additionally, antibodies against CD86 (Elabscience, Wuhan, China; cat. no. 213111) and CD206 (BD Biosciences, San Jose, CA, USA; cat. no. 565250) were utilized.

    Article Title: Glucose oxidase-mineralized scaffolds with multiple bioactivities for accelerated diabetic bone regeneration.
    Article Snippet: Total RNA from BMSCs was extracted using TRIzol, and its concentration was quantified with a Nanodrop 2000 spectrophotometer.

    Staining:

    Article Title: Glucose oxidase-mineralized scaffolds with multiple bioactivities for accelerated diabetic bone regeneration
    Article Snippet: .. After co-incubation for 24 h, the cells were stained with antibodies against CD86 (Elabscience, E-AB-F0994E) and CD206 (Elabscience, E-AB-F1135C). .. The cells stained with fluorescent antibodies were analyzed by flow cytometry (CytoFLEX S, Beckman Coulter, USA), and data were processed with FlowJo 10.8.1 (BD biosciences, USA).



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    Therapeutic effects of Que@SiO₂-GNPs in a mycoplasma-induced pneumonia model. ( A ) ELISA analysis of TNF-α, IL-6, and IL-1β levels in bronchoalveolar lavage fluid (BALF) at 1, 3, and 7 days post-treatment in Model, SiO₂ GNPs, and Que@SiO₂-GNPs groups. ( B ) H&E staining of lung tissues at day 7 to assess pathological injury across treatment groups. ( C ) Time-course H&E staining of lung tissues at days 14, 21, and 28 in the Que@SiO₂-GNPs group to evaluate tissue recovery. ( D ) Immunofluorescence staining for M1 marker iNOS (green) and M2 marker CD206 (red), with DAPI nuclear staining (blue), to assess macrophage polarization in lung tissues. ( E ) Immunohistochemical staining of SPLUNC1 expression in airway epithelium as a marker of epithelial defense restoration. ( F ) Western blot analysis and quantification of iNOS, <t>CD86,</t> and CD206 protein expression in lung tissue lysates, normalized to β-actin. Data are presented as mean ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001
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    Therapeutic effects of Que@SiO₂-GNPs in a mycoplasma-induced pneumonia model. ( A ) ELISA analysis of TNF-α, IL-6, and IL-1β levels in bronchoalveolar lavage fluid (BALF) at 1, 3, and 7 days post-treatment in Model, SiO₂ GNPs, and Que@SiO₂-GNPs groups. ( B ) H&E staining of lung tissues at day 7 to assess pathological injury across treatment groups. ( C ) Time-course H&E staining of lung tissues at days 14, 21, and 28 in the Que@SiO₂-GNPs group to evaluate tissue recovery. ( D ) Immunofluorescence staining for M1 marker iNOS (green) and M2 marker CD206 (red), with DAPI nuclear staining (blue), to assess macrophage polarization in lung tissues. ( E ) Immunohistochemical staining of SPLUNC1 expression in airway epithelium as a marker of epithelial defense restoration. ( F ) Western blot analysis and quantification of iNOS, CD86, and CD206 protein expression in lung tissue lysates, normalized to β-actin. Data are presented as mean ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001

    Journal: Journal of Inflammation (London, England)

    Article Title: Quercetin-loaded silicon dioxide–graphene nanoparticles promotes M2 macrophage reprogramming in mycoplasma-induced pneumonia

    doi: 10.1186/s12950-026-00484-y

    Figure Lengend Snippet: Therapeutic effects of Que@SiO₂-GNPs in a mycoplasma-induced pneumonia model. ( A ) ELISA analysis of TNF-α, IL-6, and IL-1β levels in bronchoalveolar lavage fluid (BALF) at 1, 3, and 7 days post-treatment in Model, SiO₂ GNPs, and Que@SiO₂-GNPs groups. ( B ) H&E staining of lung tissues at day 7 to assess pathological injury across treatment groups. ( C ) Time-course H&E staining of lung tissues at days 14, 21, and 28 in the Que@SiO₂-GNPs group to evaluate tissue recovery. ( D ) Immunofluorescence staining for M1 marker iNOS (green) and M2 marker CD206 (red), with DAPI nuclear staining (blue), to assess macrophage polarization in lung tissues. ( E ) Immunohistochemical staining of SPLUNC1 expression in airway epithelium as a marker of epithelial defense restoration. ( F ) Western blot analysis and quantification of iNOS, CD86, and CD206 protein expression in lung tissue lysates, normalized to β-actin. Data are presented as mean ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001

    Article Snippet: For AMs, cells were fixed with 4% paraformaldehyde, blocked, and stained with antibodies against CD86 (1:200, CST) and CD206 (1:200, Abcam) following the same procedure.

    Techniques: Enzyme-linked Immunosorbent Assay, Staining, Immunofluorescence, Marker, Immunohistochemical staining, Expressing, Western Blot

    Effects of Que@SiO₂-GNPs on alveolar macrophage polarization in Mycoplasma-infected rats. ( A ) Cellular uptake of FITC-labeled Que@SiO₂-GNPs in alveolar macrophages (AMs) visualized by phalloidin (red), DAPI (blue), and FITC (green) fluorescence. ( B ) ELISA analysis of Arg-1, TNF-α, and IL-6 levels in LPS-stimulated AMs treated with SiO₂ GNPs or Que@SiO₂-GNPs. ( C ) CCK-8 assay evaluating AM viability at 5, 12, 24, and 48 h after treatment. ( D – E ) Immunofluorescence staining of CD86 (M1 marker) and CD206 (M2 marker) in AMs, with DAPI counterstaining; corresponding quantitative analysis. ( F ) Flow cytometry plots showing percentages of M1 (CD86⁺) and M2 (CD206⁺) macrophages at 24 and 48 h post-treatment. Data are expressed as mean ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001

    Journal: Journal of Inflammation (London, England)

    Article Title: Quercetin-loaded silicon dioxide–graphene nanoparticles promotes M2 macrophage reprogramming in mycoplasma-induced pneumonia

    doi: 10.1186/s12950-026-00484-y

    Figure Lengend Snippet: Effects of Que@SiO₂-GNPs on alveolar macrophage polarization in Mycoplasma-infected rats. ( A ) Cellular uptake of FITC-labeled Que@SiO₂-GNPs in alveolar macrophages (AMs) visualized by phalloidin (red), DAPI (blue), and FITC (green) fluorescence. ( B ) ELISA analysis of Arg-1, TNF-α, and IL-6 levels in LPS-stimulated AMs treated with SiO₂ GNPs or Que@SiO₂-GNPs. ( C ) CCK-8 assay evaluating AM viability at 5, 12, 24, and 48 h after treatment. ( D – E ) Immunofluorescence staining of CD86 (M1 marker) and CD206 (M2 marker) in AMs, with DAPI counterstaining; corresponding quantitative analysis. ( F ) Flow cytometry plots showing percentages of M1 (CD86⁺) and M2 (CD206⁺) macrophages at 24 and 48 h post-treatment. Data are expressed as mean ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001

    Article Snippet: For AMs, cells were fixed with 4% paraformaldehyde, blocked, and stained with antibodies against CD86 (1:200, CST) and CD206 (1:200, Abcam) following the same procedure.

    Techniques: Infection, Labeling, Fluorescence, Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Immunofluorescence, Staining, Marker, Flow Cytometry